Cleaning Procedure For Biological Safety Cabinet

This may take more or less time depending on the disinfectant and the microbiological agent used.

Cleaning procedure for biological safety cabinet.

When work is completed all equipment and supplies from the bsc should be decontaminated and removed from the cabinet. It should be noted that the. Biological safety cabinet sop bsc 01. Hepa filters are defined as air cleaning devices that have a proven minimum removal efficiency of 99 97 of particles in the air equal to 0 3.

The biological safety officer will determine the type of decontamination necessary. Typically surface decontamination is adequate however there are instances such as extensive repair inside the cabinet envelope or hepa filter replacement that will require gas decontamination. Once the cabinet is empty you should allow 20 30 minutes to thoroughly decontaminate all interior work surfaces making sure to clean the back and side of the cabinet and the interior of the glass. How to clean a biological safety cabinet lafw hood.

5 1 2 dedust the biosafety cabinet daily externally with a clean dry cloth. Aseptic technique and daily decontamination will eliminate the majority of contaminants. μ microns in diameter with higher efficiency for both larger and. Spray the surfaces of all items placed into the cabinet with 70 ethanol to minimize the introduction of contaminants into the bsc.

The interior surfaces should also be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. Allow to air dry. Cleaning and disinfection 1. Put the waste from the spill into a biohazard bag inside the cabinet.

5 0 procedure 5 1 general cleaning procedure 5 1 1 ensure that the power supply of the biosafety cabinet is switched off. Remember to wear appropriate ppe and leave the biosafety cabinet on during spill cleanup. The biohazard bag should never be kept outside the cabinet. If the biological safety cabinet is turned off between aseptic processes for routine maintenance or any other reason it should be operated long enough to allow complete purging of room air from the critical area for at least 30 minutes then cleaned and disinfected before use.

How to clean a biological safety cabinet lafw hood. Hepa stands for high efficiency particulate air. In addition periodic and thorough decontamination routines including disinfecting all removable parts and surfaces is recommended by the manufacturer and is a good laboratory practice to reduce wear on the equipment and provide greater safety to you your samples and the environment. Cleaning a biosafety cabinet or bsc is an important function in terms of both containment and sterility.

To begin apply disinfect to a towel and decontaminate and remove items from the area of the spill. Afterward wipe the surface with a clean dry cloth to remove the moisture.